Get AI-powered advice on this job and more exclusive features.Direct message the job poster from Humanitas UniversityOverviewResearch Project Overview/Background: Pituitary neuroendocrine tumors (PitNETs) impact health via hormonal hypersecretion and brain invasion, with most cases lacking known driver mutations. We recently identified germline mutations in the PAM gene in PitNET patients. PAM, a multifunctional protein, regulates hormone amidation and secretion, with altered expression linked to cancer mortality.Hypothesis: PAM defects predispose to PitNETs by disrupting its non-catalytic functions, unveiling a novel disease mechanism.Aims: We aim to elucidate how PAM loss drives pituitary tumorigenesis via three approaches:Investigate how PAM mutations affect intracellular pathways and hormone secretion in tumor cell lines.Study pituitary-specific PAM loss in mice to assess tumorigenesis and systemic effects.Evaluate PAM staining as a biomarker in human PitNETs.Expected Results: Our findings will reveal PAM’s non-enzymatic roles in PitNET development, provide systemic insights, and assess PAM as a diagnostic/prognostic tool, advancing genetic testing and early diagnosis.Activities to be carried outThe work will include:Mammalian cell culture, including performing functional studies of different genetic mutations;Establishing and phenotyping mouse models.Mandatory requirementsMaster degree in Biology, Molecular Biology, Medical, Industrial, veterinary and pharmaceutical biotecnologiesPhD or equivalent;Adequate scientific and professional background to carry out the research activity described in this call.Selection CriteriaSelection criteria are predetermined by the Selection Committee. As part of the selection process, the Committee will evaluate the curriculum, titles and publications presented by the candidate and will consider:Good knowledge of the English languageExperience with molecular biology techniques and mammalian cell cultureExperience in working with mouse modelsRequired documents for your applicationCover letter explaining why you want to join the teamUpdate CVContact information of three refereesOn the basis of the profiles received and in the terms indicated by this notice, Humanitas will activate the selection process by inviting suitable candidates to participate in selection interviews, after verification of the cv and qualifications, which will be held in presence or in telematic mode.The notice is addressed to candidates in compliance with Dlgs 198/2006 and Dlgs 215/2003 and 216/2003. Interested persons are invited to read the privacy notice ex art. 13 and 14 of EU Reg. 2016/679.
#J-18808-Ljbffr